Green Castle Estate has a strong precedent for applied research in agriculture, archeology and the biological and ecological sciences. Over the years a variety of partnerships were cultivated with entities such as the University of the West Indies, the Jamaica Coconut Board, and the Jamaica Agriculture Development Foundation to research and address issues at Green Castle Estate that have much broader relevance both within Jamaica and abroad. This precedent created a strong foundation from which to launch additional efforts under Greencastle Tropical Study Center.

Some examples of research and class work conducted at Green Castle include:

• University of Minnesota Tropical Water Quality Class taught by Dr. Jim Perry in conjunction with University of the West Indies Teaching Assistants Hugh Small and Sacha Todd.
• A collaborative partnership between the University of the West Indies and Murray State University resulted in archeological digs to study a number of Taino community sites at Greencastle Estate over a five-year period, from 1999 to 2003

• A Sustainability Memo was produced in 2008 by University of Minnesota Graduate student Renee Maas.
• The Jamaica Coconut Board established five test plots of coconut trees on Green Castle from 1995 to 1998. The data from these plots are assisting the Board in the fight against Lethal Yellowing, a fatal coconut palm disease.
• With assistance from the Jamaica Agriculture Development Foundation Sea Island organic cotton was grown at Green Caste Estate in 2005.
• Ornithological research at Greencastle Tropical Study Center was most recently conducted in early 2006, when a team from Humboldt State University conducted surveys throughout the property during a two-month period.
• A number of organic vegetables have been grown as test crops with the support and assistance of JOAM, the Jamaica Organic Agricultural Movement, www.JOAMltd.org.